Research focus The overall goal of research of the Cell and Tissue Regeneration team is to investigate the properties of human stem cells that are relevant to their utility in biomedicine. There is a particular focus on pluripotent stem cells, both embryonic and induced, but adult stem cell types are also studied. The biological phenomena that are being studied mainly include: a) genomic stability of stem cells because of its importance to safety in potential clinical applications, b) role of cell cycle regulators and non-coding RNAs in establishing and maintenance of differentiated phenotypes and also in pluripotency, and c) effects of extracellular biodomains on behavior of stem cells, with an emphasis on their contribution to the establishment of niche environments. Great effort is also dedicated to the understanding of stem cell-to-extracellular matrix interactions and to the development of methodologies for supporting growth of cells in engineered scaffolds as well as differentiation of stem cells towards defined somatic cell lineages (e.g. airway epithelia).
• Evaluation of the effects of distribution of regulatory biodomains on the behaviors of cells with use of the “molecular lawn” approach.
• Establishment of clinical grade (GMP quality) lines of human embryonic stem cells and their functional evaluation.
• Understanding and manipulating biological properties of human pluripotent stem cells that are relevant to their application in clinics.
• Karolinska Institutet, Stockholm, Sweden
• University of Newcastle, Newcastle, UK
• Stem Cell Institute, MRC, Cambridge, UK
• Oslo University Hospital, Oslo, Norway
Offered services and expertise Live cell imaging microscopy; transmission and scanning electron microscopy; stem cell phenotyping; establishment, propagation, and differentiation of human pluripotent stem cells; mass spectrometry-based cell fingerprinting.
DOLEŽALOVÁ, D., MRÁZ, M., BÁRTA, T., PLEVOVÁ, K., VINARSKÝ, V., HOLUBCOVÁ, Z., JAROŠ, J., DVOŘÁK, P., POSPÍŠILOVÁ, Š., HAMPL, A. MicroRNAs Regulate p21 Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells. Stem Cells. 2012, 30(7), 1362-1372.
BARTA, T., PESKOVA, L., HAMPL, A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Sci Rep. 2016, 6:36625.
STREIT, L., JAROS, J., SEDLAKOVA, V., SEDLACKOVA, M., DRAZAN, L., SVOBODA, M., POSPISIL, J., VYSKA, T., VESELY, J., HAMPL, A. A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting. Plast Reconstr Surg. 2017, 139:670e-682e.
Contribution to understanding of why and how human embryonic stem cells may develop abnormalities to their genome. These findings create theoretical grounds for development of strategies for safe clinical application of stem cells.
Unraveling of non-coding RNAs that contribute to establishment of cell properties that are typical for pluripotent stem cells. These findings may help to develop strategies for driving cells towards particular functional somatic phenotypes.
Development of methodologies for authentication of cells using mass spectrometry and non-linear analysis by neural networks.
INTERNATIONAL CLINICAL RESEARCH CENTER
OF ST. ANNE’S UNIVERSITY HOSPITAL BRNO